Time will prolong time, and life will serve life.

/ Albert Camus /


Cheslav Ivanovich Chenzinsky and Dmitry Leonidovich Romanowsky. have given rise to stain techniques of biological preparations with mixture of azur B, methylene blue and eosin Y.

The priority in polychrome staining of blood smears with mixture of azur B, methylene blue and eosin Y belongs to Romanowsky D.L..

Нis works initiated application and perfection of staining technique, which is used all over the world 120 years.

It is so called «Romanowsky stain» or «Romanowsky type stain».

Further a great number of doctors all over the world have taken part in perfection of a technique which is applied practically in each medical laboratory.

Works of Jenner, Leishman, May and Günvald, Nocht, Giemsa, Wright, Pappeheim and group of the authors who have developed ICSH reference technique of Romanowsky type staning, are most known.

In connection with the 125 anniversary of the publication of the article  D. L. Romanowsky, "On the structure of malaria parasites" (VRACH, 1890, number 52, 1171-1173.), where he firstly described the methods and results of staining of blood preparations with polychrome effect, are placed on the site  file with the original article, article translated into Russian with the modern spelling and an English translation.


In Biotechnic & Histochemistry Journal 18.01.2016 published an article by AV Bezrukov «Romanowsky staining, the Romanowsky effect and thoughts on the question of scientific priority».

The text of article, after on line publication by Biotechnic & Histochemistry Journal, is posted on the website according to AGREEMENT IN RELATION TO COPYRIGHT - Bezrukov - 28-10-2016 (Standard publication route).

The article is about the history of the discovery and the Romanowsky effect and first variants of Romanowsky staining techniques. Proposed updated versions of the definitions of " Romanowsky effect " and " Romanowsky staining." is determined on the basis of the Romanowsky effect primacy.

Both formal priority for polychrome staining of blood preparations by a solution of eosin plus methylene blue with its oxidation products (azures B and A) and the development of this technique belong to D. L. Romanowsky. His work showed the possibility of using simple methods to obtain polychrome blood preparations with high contrast and resolution that were suitable for identifying the structure of malaria parasites. This was the starting point for the development of polychrome staining techniques and subsequent scientific microscopy examinations for a long sequence of researchers.

To cite this article: AV Bezrukov (2017): Romanowsky staining, the Romanowsky effect and thoughts on the question of scientific priority, Biotechnic & Histochemistry, DOI: 10.1080/10520295.2016.1250285

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